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Mice deficient for cathepsin L CTSL show epidermal hyperplasia due to a hyperproliferation of basal keratinocytes.
Admin29.07.2021

The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

Paraffin sections were stained in haematoxylin and eosin HE or by immunohistochemistry for the proliferation marker Ki67 Ki67, brown nuclear staining and the differentiation markers cytokeratin 10 K10, brown staining and transglutaminase TG, brown staining.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

About half of the offspring mice carried the transgene.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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Inhibitor studies have suggested that the EGF-receptor can be degraded by a cathepsin L-like protease and that CTSL is involved in degradation of the insulin-like growth factor binding protein-3.
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Admin16.07.2021

The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

63 kb] of the human growth hormone gene.
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Subsequently, dermis and epidermis were carefully separated.
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